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Aushon SearchLight Chemiluminescent Assay Kits are stock 4- to 9-plex kits for simultaneous measurement of popular combinations and species of cytokine, chemokine, angiogenesis and matrix metalloproteinase targets. The kits are complete, validated sets of spotted microplate and reagents that are as simple to use as traditional ELISA kits. Each kit consists of the multiplex pre-spotted plate, wash and dilution buffers, biotinylated antibody cocktail, streptavidin-HRP conjugate and chemiluminescent substrate solutions.
The kits allow researchers with access to an appropriate CCD imaging system and array software (details below) to quantify multiple proteins per well in a 96-well plate, generating cytokine and biomarker profiles for important samples. The chemiluminescent arrays offer accurate, reproducible data for up to 40 samples per 96-well plate (if tested in duplicate) in just 3 to 5 hours. Each new lot of array plates is quality-tested for spotting consistency and specificity, to ensure there is no cross-reactivity or non-specific binding resulting from new antibody combinations.
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Highlights:
- Sensitive multiplex analysis – obtain more information per limited amount of valuable sample. Measure four to nine proteins per microplate well (i.e., per 10-50 µl sample), detecting even low-abundance proteins with the sensitivity afforded by quantitative chemiluminescence
- Accurate and quantitative -- Generate pg/ml protein values from each unknown sample. Lot-specific kit validation ensures that data are quantitative in the specified assay range
- Optimized multiplexes -- spotted and detection antibody concentrations are optimized to provide the best assay range for each analyte with typical biological samples.
- Sample compatibility – SearchLight Technology has been used with a variety of sample types, including culture supernatant, serum, plasma, BAL, CSF, crevicular fluid, amniotic fluid, nasal wash, cell lysate, peritoneal lavage, synovial fluid, breath condensate, brain microdialysate and various tissue homogenates.
- Fast and accurate imaging and analysis – CCD imaging of SearchLight Assays is completed in ~1 to 5 minutes, depending on the imaging system use, making it easy to share instrumentation and analyze multiple plates per day. SearchLight Array Analysis Software is validated for the technology (see below). The image from each plate can be saved for a permanent record of raw experimental data.
Instrument and Software Requirements:
Because multiple assays are conducted within each well, a CCD imaging system and specialized array software are required for SearchLight Chemiluminescent Array analysis. Several commercially available systems are capable of SearchLight Array detection. At a minimum, the system must include an enclosed CCD system with a 12-bit camera (16-bit preferred) that is cooled to a minimum of -25°C. Instruments that work well for SearchLight Chemiluminescent Assays include Fuji LAS-3000 and LAS-4000, GE Healthcare LEADSeeker and PerkinElmer ViewLux Imagers. SearchLight Array Analyst Software is specifically optimized for analysis of quantitative protein arrays. Trial software is available.
Aushon's complete portfolio protein biomarker research products and services includes:
- Aushon 2470 Arrayer: The industry’s most reliable microarray printer provides researchers with a single instrument for genomic and proteomic microarray applications, with unmatched sample and substrate flexibility and unequaled accuracy.
- Custom Microarray Printing: Unmatched versatility and flexibility means printing virtually any sample on any substrate – with unequaled reproducibility, accuracy and rapid turnaround.
- SearchLight Sample Testing Service: A convenient, custom service that allows researchers to submit samples for quantitative analysis in custom SearchLight Biomarker Arrays.
- Custom Arrays and Assay Development: Select from a menu of more than 300 proteins to design an array consisting of 2 to 16 proteins per well.
Ordering Information:
For ordering information, please email us at sales@aushon.com, or contact your Aushon SearchLight Account Representative at 978-436-6464
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